![]() The immature DCs capture and internalize specific antigens, followed by expression of co-stimulatory molecules and maturation, and then present the processed antigens to stimulate T cell differentiation and B cell production, which play important roles in host immune response. The recognition function of DCs in innate immunity is based on their ability to recognize pathogenic microorganisms by special receptors, such as receptors for C-type lectins (DC-SIGN, DEC-205), toll-like receptors (TLR), receptors for the Fc portion of antibodies (FcR), complement receptors (CR3 and CR4), as well as CD11c, which belong to the heterodimeric receptor family of β2-integrins. ĭendritic cells (DCs), belonging to the family of antigen- presenting cells (APCs), play a critical role in both innate immunity and adaptive immunity. plantarum has been studied in great detail for its immunomodulatory effects and adjuvant-like actions. plantarum strains are able to colonize more efficiently in the intestinal tract, which is the first step for efficient antigen production and necessary for subsequent antigen processing in host cells. To date, a large number of protective antigens have been previously delivered by LAB strains, mainly including Lactococcus lactic ( L. This being the case, the mucosal delivery vectors have been drawing more attention recently due to their orally administrated method of immunization.Īmong these vectors, one of the probiotic strains, lactic acid bacteria (LAB), has raised a lot of interest due to its beneficial properties for human health. Besides, the traditional injection approach makes it inconvenient for application in the field, especially in poultry production, for example. However, this strategy may render the DNA unstable in some cases. KeywordsĭNA vaccination, a type of third-generation vaccine (also called a nucleic acid vaccine or gene vaccine), primarily depends on the injection of a eukaryotic plasmid containing DNA into host cells using an intramuscular strategy. plantarum strain which could increase the ability to deliver eukaryotic expression plasmid to host cells, indicating a promising approach for vaccine study. In conclusion, this study developed a novel DC-targeting L. In addition, the CD11c-targeting strain significantly promoted the differentiation and maturation of DCs, the differentiation of IL-4 + and IL-17A + T helper (Th) cells in MLNs, as well as production of B220 + IgA + B cells in the PP. Similar results were also observed in an in vivo study, which showed that oral administration resulted in efficient expression of GFP in both Peyer’s patches (PP) and mesenteric lymph nodes (MLNs) within 7 days after the last administration. After incubation with BMDCs, the 409-aCD11c strain harboring a eukaryotic vector pValac-GFP could lead to more efficient expression of GFP compared with wild-type strains shown by flow cytometry analysis, indicating the enhanced translocation of pValac-GFP from L. plantarum strain, named 409-aCD11c, could adhere and invade more efficiently to bone marrow-derived DCs (BMDCs) in vitro due to the specific interaction between scFv-CD11c and CD11c located on the surface of BMDCs. plantarum strain with surface-displayed variable fragments of anti-CD11c, single-chain antibody (scFv-CD11c). ![]() Considering to the critical role of dendritic cells in stimulating host immune response, in this study, we constructed a novel CD11c-targeting L. plantarum) has been used extensively for vaccine delivery. The lactic acid bacteria species Lactobacillus plantarum ( L.
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